pysted.base.Objective

class pysted.base.Objective(**kwargs)

This class implements the microscope objective component.

Parameters:

parameters – One or more parameters as described in the following table, optional.

Parameter

Default [1]

Details

f

2e-3

The objective focal length (m).

n

1.5

The refractive index.

na

1.4

The numerical aperture.

transmission

488: 0.84, 535: 0.85, 550: 0.86, 585: 0.85, 575: 0.85

A dictionary mapping wavelengths (nm), as integer, to objective transmission factors (ratio).

Objective.get_transmission(lambda_)