pysted.base.Objective
- class pysted.base.Objective(**kwargs)
This class implements the microscope objective component.
- Parameters:
parameters – One or more parameters as described in the following table, optional.
Parameter
Default [1]
Details
f
2e-3
The objective focal length (m).
n
1.5
The refractive index.
na
1.4
The numerical aperture.
transmission
488: 0.84,
535: 0.85,
550: 0.86,
585: 0.85,
575: 0.85
A dictionary mapping wavelengths (nm), as integer, to objective transmission factors (ratio).
Objective.get_transmission
(lambda_)