pysted.raster.raster_func_dymin

pysted.raster.raster_func_dymin(self, datamap, acquired_intensity, pixel_list, ratio, rows_pad, cols_pad, laser_pad, prob_ex, prob_sted, returned_photons, scaled_power, pdt_roi, p_ex_roi, p_sted_roi, bleach, bleached_sub_datamaps_dict, seed, bleach_func, sample_func, steps)

Implements the raster scanning and photobleaching of DyMIN microscopy

Parameters:

  • datamap – The datamap on which the acquisition is done, either a Datamap object or TemporalDatamap

  • acquired_intensity – 2D array to store the acquired intensity

  • pixel_list – 2D array with the position of the pixels to be acquired

  • ratio – The ratio of the pixel size to the datamap pixel size

  • rows_pad – The number of rows to pad the datamap

  • cols_pad – The number of columns to pad the datamap

  • laser_pad – The number of pixels to pad the laser

  • prob_ex – 2D array with the probability of survival of the fluorophores under excitation

  • prob_sted – 2D array with the probability of survival of the fluorophores under sted

  • pdt_roi – 2D array with the pixel dwell time

  • p_ex_roi – 2D array with the excitation power

  • p_sted_roi – 2D array with the sted power

  • bleach – Boolean to indicate if the sample should be bleached

  • bleached_sub_datamaps_dict – Dictionary with the different parts of the datamap to be bleached

  • seed – Seed for the random number generator

  • bleach_func – Function to bleach the sample

  • sample_func – Function to sample the sample

  • steps – List with the different steps of the acquisition