pysted.base.Detector.get_detection_psf

Detector.get_detection_psf(lambda_, psf, na, transmission, datamap_pixelsize)

Compute the detection PSF as a convolution between the fluorscence PSF and a pinhole, as described by the equation from [Willig2006].

The pinhole radius is determined using the n_airy, the fluorescence wavelength, and the numerical aperture of the objective.

Parameters:

  • lambda – The fluorescence wavelength (m).

  • psf – The fluorescence PSF that can the obtained using get_psf().

  • na – The numerical aperture of the objective.

  • transmission – The transmission ratio of the objective for the given fluorescence wavelength lambda_.

  • datamap_pixelsize – The size of a pixel in the simulated image (m).

Returns:

A 2D array.