pysted.base.Microscope.cache

Microscope.cache(datamap_pixelsize, save_cache=False)

Compute and cache the excitation and STED intensities, and the fluorescence PSF.

These intensities are computed with a power of 1 W such that they can serve as a basis to compute intensities with any power.

Parameters:

  • datamap_pixelsize – The size of a pixel in the simulated image (m).

  • save_cache – A bool which determines whether or not the lasers will be saved to allow for faster load times for future experiments

Returns:

A tuple containing:

  • A 2D array of the excitation intensity for a power of 1 W;

  • A 2D array of the STED intensity for a a power of 1 W;

  • A 2D array of the detection PSF.